Dr. Jennifer Hurley received her B.S. from Juniata College in 2004 in molecular biology. She did her Ph.D. at Rutgers/UMDNJ with Drs. Nancy Woychik and Masayori Inouye, studying the function of Toxin-Antitoxin modules in bacteria. She was recognized by the American Society for Biochemistry and Molecular Biology for excellence in research for her study of the HigBA toxin-antitoxin module. Jennifer did her Postdoctoral fellowship at the Geisel School of Medicine at Dartmouth with Drs. Jay Dunlap and Jennifer Loros, investigating the relationship between the core proteins and the output of the circadian clock in Neurospora. Her Fellowship was funded by the Ruth Kirschstein National Research Service Award from the National Institute of General Medical Sciences and she received a Perkins award for her contributions to Neurospora research. Dr. Hurley joined the Department of Biological Sciences at Rensselaer Polytechnic Institute in 2015 as an Assistant Professor. Dr. Hurley's research focus is on the fundamental mechanisms underlying circadian rhythms. Circadian rhythms are an important component in understanding how organisms function within the photoperiodic world that we live in; defects in the circadian clock or disruptions in circadian rhythms are linked to a wide range of sleep, metabolic and psychological disorders in humans. Her lab investigates the relationship between the core clock mechanism and the output that the clock controls using a combination of molecular genetics and biochemical techniques as well as a biostatistical/computational approach using whole genome scale data.
B.S. Molecular Biology and Politics, Juniata College (2004); Ph.D. Molecular Genetics, Microbiology and Immunology, Rutgers/UMDNJ (2009); P.Ph.D. Genetics and Biochemistry, Dartmouth (2015)
Focus AreaCircadian Rhythms, Neurospora, Macrophages, Immunology, Protein Structure/Function Relationships, Systems Biology, Ecology
Selected Scholarly WorksMost recent work listed below. Full Bibliography at http://www.ncbi.nlm.nih.gov/sites/myncbi/jennifer.hurley.1/bibliography/47796157/public/?sort=date&direction= descending
Pelham JF, Mosier AE, Hurley JM. (2018) Methods Enzymol. 611, 503-529. Characterizing Time-of-Day Conformational Changes in the Intrinsically Disordered Proteins of the Circadian Clock.
Cannon, W.R., Zucker, J.D., Baxter, D.J., Kumar, N., Baker, S.E., Hurley, J.M. and Dunlap, J.C. (2018) Processes, 6(6), 63 Prediction of Metabolite Concentrations, Rate Constants and Post-Translational Regulation Using Maximum Entropy-Based Simulations with Application to Central Metabolism of Neurospora crassa
Coldsnow, K. D.G, Relyea, R. A., and Hurley, J. M. (2017) Evolution to environmental contamination ablates the circadian clock of an aquatic sentinel species. Ecology and Evolution Oct 28;7(23):10339-10349
Hughes, M.E. … Hurley, J.M. … Hogenesch, J.B. (2017) Guidelines for genome-scale analysis of circadian rhythms. JBR Nov 3;32(5):380-393
De Los Santos, H., Collins, E.J., Hurley, J.M., Bennett, K.P. (2017) Circadian Rhythms in Neurospora Exhibit Biologically Relevant Driven and Damped Harmonic Oscillations, In Press, Aug 20 455-463
Dekhang R., Wu C., Smith K.M., Lamb T.M., Peterson M., Bredeweg E.L., Ibarra O., Emerson J.M., Karunarathna, N., Lyubetskaya A., Azizi E., Hurley J.M., Dunlap J.C., Galagan J.E., Freitag M., Sachs M.S., and Bell-Pedersen D. G3 in press The Neurospora Transcription Factor ADV-1 Transduces Light Signals, and Temporal Information to Control Rhythms in Expression of Cell-Fusion Genes.
Hurley J.M.§ Loros J.J., Dunlap J.C§. (2016) Trends in the Biochemical Sciences Oct;41(10):834-46. Circadian Oscillators: Around the Transcription-Translation Feedback Loop and on to Output.
Conrad, K, Hurley, J.M., Widom, J, Ringelberg, C, Loros, J.J., Dunlap, J.C. and Crane B.R. (2016) European Molecular Biology Organization Aug 1;35(15):1707-19. Structure of the Frequency-Interacting RNA Helicase: a protein interaction hub for the circadian clock.
Hurley J.M., Loros J.J., Dunlap J.C. (2016) Fungal Genetics and Biology May;90:39- 43. The circadian system as an organizer of metabolism.
Hurley J.M., Dasgupta A., Andrews P., Crowell A.M., Ringelberg C., Loros J.J., Dunlap J.C. (2015) G3 (Bethesda) 6;5(10):2043-9. A Tool Set for the Genome Wide Analysis of Neurospora crassa by RT-PCR.
Hurley, J.M., Loros J.J., Dunlap, J.C. Methods in Enzymology Circadian Rhythms and Biological Clocks (Amita Segal, ed.) (2015) Dissecting the Mechanisms of the Clock in Neurospora.
Hurley J.M.*, Dasgupta A.*, Emerson J.M., Zhou X., Ringelberg C.S., Knabe N., Lipzen A., Lindquist E., Daum C., Barry K., Grigoriev I.V., Smith K., Galagan J., Bell-Pedersen D., Freitag M., Cheng C., Loros J.J., Dunlap J.C. (2014) PNAS 111 16995-17002 Analysis of clock regulated genes in Neurospora reveals widespread post-transcriptional control of metabolic potential *authors contributed equally
Fuller, K.K.*, Hurley, J.M.*, Loros, J.J., Dunlap, J.C. *authors contributed equally. The Mycota Vol. XIII: Fungal Genomics, 2nd ed. 2014 (Minou Nowrousian, ed.) Photobiology and Circadian Clocks in Neurospora.
Hurley, J.M., and Dunlap, J.C. (2013). Nature 495, 57- 58. Cell biology: A fable of too much too fast.
Hurley, J.M., Larrondo, L.F., Loros, J.J., and Dunlap, J.C. (2013). Molecular cell 52, 832-843. Conserved RNA helicase FRH acts nonenzymatically to support the intrinsically disordered Neurospora clock protein FRQ.
Hurley, J.M., Chen, C.H., Loros, J.J., and Dunlap, J.C. (2012). G3 (Bethesda) 2, 1207-1212. Light-Inducible System for Tunable Protein Expression in Neurospora crassa.
Rothenbacher, F.P., Suzuki, M., Hurley, J.M., Montville, T.J., Kirn, T.J., Ouyang, M., and Woychik, N.A. (2012). J Bacteriology 194, 3464-3474. Clostridium difficile MazF toxin exhibits selective, not global, mRNA cleavage.
Hurley, J.M., Cruz, J.W., Ouyang, M., and Woychik, N.A. (2011). The Journal of Biological Chemistry 286, 14770-14778. Bacterial toxin RelE mediates frequent codon-independent mRNA cleavage from the 5' end of coding regions in vivo.
Arbing, M.A., Handelman, S.K., Kuzin, A.P., Verdon, G., Wang, C., Su, M., Rothenbacher, F.P., Abashidze, M., Liu, M., Hurley, J.M., et al. (2010). Structure 18, 996-1010. Crystal structures of Phd-Doc, HigA, and YeeU establish multiple evolutionary links between microbial growth-regulating toxin-antitoxin systems.
Hurley, J.M., and Woychik, N.A. (2009). The Journal of biological chemistry 284, 18605-18613. Bacterial toxin HigB associates with ribosomes and mediates translation-dependent mRNA cleavage at A-rich sites.
Kinzy, T.G., De Stefano, L.A., Esposito, A.M., Hurley, J.M., Roy, R., Valentin-Acevedo, A.J., Chang,K.H., Davila, J., Defren, J.M., Donovan, J., et al. (2008). Biochem Mol Biol Educ 36, 1-8. A birth-to-death view of mRNA from the RNA recognition motif perspective.
Arbing, M.A., Abashidze, M., Hurley, J.M., Zhao, L., Janjua, H., Cunningham, K., Ma, L.C., Xiao, R., Liu, J., Baran, M.C., Acton, T.B., Rost, B., Inouye, M., Woychik, N.A., Montelione, G.T., Hunt, J.F.. Northeast Structural Genomics Consortium (NESG) Published online in Protein Data Base Crystal structure of the bacterial antitoxin HigA from Escherichia coli at pH 8.5. Northeast Structural Genomics TARGET ER390.
Hurley J.M., Jankowski, M.S., De Los Santos, H., Crowell A.M., Fordyce S.B., Zucker, J.D., Kumar, N., Purvine, S.O., Robinson, E.W., Shukla, A., Zink, E., Cannon, W.R., Baker, S.E., Loros, J.J., Dunlap, J.C. (2018). Circadian proteomic analysis uncovers mechanisms of post-transcriptional regulation in metabolic pathways. Cell Systems